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. 2016 Mar 28;11(3):e0150026. doi: 10.1371/journal.pone.0150026

Fig 3. EZH2 is a direct target of miR-138 in osteosarcoma cells.

Fig 3

(A) Sequence of the miR-138-binding site within the human EZH2 3′-UTR and the mutated EZH2 3′-UTR sequence. (B) Luciferase assay on MG-63 cells, which were co-transfected with miR-NC or miR-138 and a luciferase reporter containing the full length of EZH2 3′-UTR(WT) or a mutant(Mut). Luciferase activities were measured 24 hours post transfection. (C) The expression levels of EZH2 were decreased in cells with miR-138 overexpression by western blotting (D) Spearman′s correlation analysis was used to determine the correlation between the expression levels of EZH2 and miR-138 in 20 pairs of adjacent normal and osteosarcoma samples.