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. 2016 Mar 28;11(3):e0152346. doi: 10.1371/journal.pone.0152346

Fig 3. Endogenous TRIB1 is undetectable by straight western blot.

Fig 3

A, HeLa, 293T and ASMC lysates were lysed in IP buffer and resolved by SDS-PAGE. Purified GST-TRIB1 fusion (GT1) protein was included as positive control. B, HeLa and 293T cells treated for 48 h with a TRIB1 (ASO1) or a control (NT) antisense oligonucletide were lysed in IP buffer supplemented with protease and phosphatase inhibitor cocktails, resolved by SDS-PAGE and analyzed by western blotting using 2 distinct TRIB1 antibodies. Cell lines stably transduced with TRIB1 and lysed under the same conditions were used as positive control. TRIB1 bands (indicated by side bars) had apparent masses ranging from 43–48 kDa. * indicates residual TUBB signal.