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. 2016 Mar 28;11(3):e0152346. doi: 10.1371/journal.pone.0152346

Fig 4. TRIB1 is unstable in HeLaT1 and 293T1 in vivo but stable in vitro.

Fig 4

A, stably integrated pools of cells were treated with CHX or ACTD for 5 h and analyzed by western blot using TRIB1(TRIB1g (goat) or TRIB1r (rabbit)) and TUBB specific antibodies. Data are representative of 3 experiments. Quantification presented is for the TRIB1g and TUBB only. B, TRIB1 stable cells were lysed in IP buffer with no additives, diluted in assay buffer and incubated at 37°C for the indicated time. Western blot was performed with the indicated antibodies.