Fig 6. Proteasome inhibitors increase TRIB1 but do not prevent TRIB1 destabilization in stably transduced cells.

Cells were treated for 5 h with vehicle, CHX or ACTD, in combination with MG132 (20 μM) or Bortezomib (BTZ, 10 μM), as indicated. A, Lysates were subjected to western blot using the indicated antibodies. B, mRNA was isolated and quantified by qRT-PCR for TRIB1. Values are normalized to vehicle alone (DMSO) and represent the mean of biological triplicates ± S.D.