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. 2016 Mar 28;11(3):e0152346. doi: 10.1371/journal.pone.0152346

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© 2016 Soubeyrand et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 10 — Top, schema of the deletions and insertions introduced in FLAG-tagged TRIB1. Bottom, immunofluorescence analysis of HeLa cells transfected for 48 h with the indicated TRIB1 mutants. FLAG tagged TRIB1 expressing cells were fixed, permeabilized and examined by confocal microscopy using an antibody specific for the FLAG epitope and an anti-mouse secondary (Alexa 647 goat anti-mouse, Molecular Probes). Nuclei were counterstained with Hoechst 33342.