Table 1. Statistics of the multiplex PCR and 5’RACE libraries.

Sample	S01-M-1	S01-M-2	S02-M-1	S03-M-1	S01-R-1	S02-R-1	S02-R-2	S03-R-1
Total reads	8,126,815	14,588,545	12,612,786	39,754,077	24,555,544	24,450,175	24,204,014	46,409,300
Clean readsa	7,955,514	14,293,710	12,396,695	37,797,350	18,559,742	18,590,382	17,739,268	40,325,274
PE reads mergedb	7,934,499	14,251,076	12,365,441	37,447,896	16,755,290	17,713,913	15,704,110	37,861,325
VJ alignmentc	7,821,564	14,054,378	12,150,255	36,496,389	10,763,131	13,726,534	12,427,498	29,851,422
Correct ORF readsd	7,439,792	13,448,043	11,122,904	34,838,414	9,585,671	12,638,442	11,460,936	28,265,147
Effective readse	6,880,594	12,664,905	10,398,513	27,823,441	9,585,671	12,019,778	11,023,329	26,067,794
Effective reads Rate	84.67%	86.81%	82.44%	69.99%	39.04%	49.16%	45.54%	56.17%
Unique CDR3 nucleotidesf	148,262	204,041	170,733	1,061,044	265,458	156,655	81,008	541,446
Unique CDR3 peptidesg	138,831	188,164	158,991	858,389	247,403	147,035	76,636	483,083
Simpson’s diversity index (CDR3)	0.9961	0.9949	0.9288	0.9878	0.9881	0.9985	0.9985	0.9828

^aClean reads refer to reads after filtering the low-quality sequences

^bPE merged reads refer to paired-end reads merged filtered by the sequences with a stop codon, incorrect CDR3 length, and mapped to pseudo genes

^cVJ alignment refers to reads mapped to V and J genes

^dCorrect ORF reads were determined to have the CDR3 region

^eEffective reads were filtered by the low-frequency sequences

^fUnique CDR3 nucleotide represents a non-redundant fragment (CDR3) of nucleotide

^gUnique CDR3 peptide represents a non-redundant fragment (CDR3) of amino acids.