Figure 4.

Corilagin inhibits migration and invasion of CCA cells. CCA cells were analysed for wound healing and cell invasion following treatment with corilagin. (A) Light microscopic images of QBC939 cells scratched with a pipette tip when they were confluent (upper panel), and after treatment with 40 μmol/l corilagin for 48 h (lower panel). (B and D) Light microscopic images of CCA cells, which have migrated through pores of the polycarbonate filters to the lower membrane surface after treatment with 40 μmol/l corilagin for 12 h. Cells were stained with crystal violet. (C and E) Cell migration (cells/field) of CCA cells, as assessed by the transwell migration assay. The results shown are representative of three independent experiments and represent means ± SD. ^*P<0.01, ^#P<0.05 versus control cells.