Table 2. Weight and Quantified gait parameters in spast^wt/wt, spastin^N384K/wt, spastin^N384K/N384K mice at 1 year old.

Values are shown for the left hind limb unless otherwise stated; very similar results were obtained for the right hind limb. p-values for the effect of gender and genotype were calculated using two-way ANOVA. To correct for multiple testing, the alpha value for significance was calculated by dividing 0.05 by 8, using a highly conservative assumption of 8 independent tests (many of the tests are in reality related), to give α = 0.00625. spast^wt/wt n = 10 (4 male, 6 female), spastin^N384K/wt n = 16 (7 male, 9 female), spastin^N384K/N384K n = 10 (6 male, 4 female).

Parameter	Mean ± S.E.M. for: spastwt/wt spastinN384K/wt spastinN384K/N384K	p-value for effect of Gender	p-value for effect of genotype
Weight (g)	44.0 ± 1.06	0.08	0.0008
	42.8 ± 1.30
	36.4 ± 2.23
Mean stride duration (s)	0.31 ± 0.011	0.96	0.005
	0.32 ± 0.007
	0.36 ± 0.007
Mean swing phase duration (s)	0.09 ± 0.004	0.83	< 0.0001
	0.10 ± 0.003
	0.14 ± 0.01
Mean stance phase duration (s)	0.22 ± 0.01	0.88	0.79
	0.22 ± 0.02
	0.21 ± 0.01
Mean braking duration in stance phase (s)	0.04 ± 0.0005	0.33	0.98
	0.04 ± 0.002
	0.04 ± 0.003
Mean propulsion duration in stance phase (s)	0.18 ± 0.0005	0.61	0.82
	0.18 ± 0.005
	0.17 ± 0.004
Mean stride length (cm)	5.7 ± 0.15	0.92	0.006
	5.8 ± 0.08
	6.4 ± 0.04
Mean hindlimb stance width (cm)	2.8 ± 0.11	0.09	0.06
	2.7 ± 0.03
	3.0 ± 0.19
Mean gait symmetry ratio (1 = entirely symmetrical)	1.01 ± 0.01	0.42	0.64
	1.00 ± 0.006
	1.00 ± 0.005