Table 4. Quantified gait parameters in spast^wt/wt, spastin^N384K/wt, spastin^N384K/N384K mice at 4 months old.

Values are shown for the left hind limb unless otherwise stated. p-values for the effect of genotype were calculated using one-way ANOVA. To correct for multiple testing, the alpha value for significance was calculated by dividing 0.05 by 8, using a highly conservative assumption of 8 independent tests (many of the tests are in reality related), to give α = 0.00625. N = 3 mice in each group.

Gait Parameter	Mean ± S.E.M. for: spastwt/wt spastinN384K/wt spastinN384K/N384K	p-value for effect of genotype
Mean stride duration (s)	0.30 ± 0.005	0.002
	0.32 ± 0.006
	0.38 ± 0.01
Mean swing phase duration (s)	0.11 ± 0.006	0.088
	0.11 ± 0.003
	0.12 ± 0.004
Mean stance phase duration (s)	0.20 ± 0.01	0.0014
	0.21 ± 0.02
	0.25 ± 0.01
Mean braking duration in stance phase (s)	0.033 ± 0.003	0.16
	0.036 ± 0.006
	0.046 ± 0.009
Mean propulsion duration in stance phase (s)	0.16 ± 0.002	0.0003
	0.17 ± 0.002
	0.21 ± 0.006
Mean stride length (cm)	5.5 ± 0.09	0.0012
	5.7 ± 0.1
	6.8 ± 0.2
Mean hindlimb stance width (cm)	2.6 ± 0.07	0.52
	3.0 ± 0.29
	2.9 ± 0.18
Mean gait symmetry ratio (1 = entirely symmetrical)	1.02 ± 0.02	0.28
	1.04 ± 0.01
	1.08 ± 0.04