Table 1. Primers used for genome editing and subsequent testing in this study.

Primers	Sequence		Application
P1		GTGACGGAAGATCACTTCGCAG	cat-sacB fragment construction
P2		ATCAAAGGGAAAACTGTCCATATGC	cat-sacB fragment construction
KtesAF		CCGACGGACTTCTTAAGATGATGAACTTCAACAATGTTTTCCGCTGGCATATGACTCATAAAGCAACGGAGATCCTGACAGGTAAAGTTATGCAAAAATC$\mathrm{TCCTGGTGTCCCTGTTGATA}$	tesA deletion
KtesAR		GATTTTTGCATAACTTTACCTGTCAGGATCTCCGTTGCTTTATGAGTCAT$\mathrm{ATAGATACATCAGAGCTTTTACGAG}$	tesA deletion
KtesBF		ATCACGCATTTCTGCCTGTAATTAGCCCGTAATTCAGACCATTGCACCCAAAAAATAGCCGGAGGTGAAAACCGTCCGGCTGTTTTTTGCAGTGCTTGTT$\mathrm{TCCTGGTGTCCCTGTTGATA}$	tesB deletion
KtesBR		AACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTT$\mathrm{ATAGATACATCAGAGCTTTTACGAG}$	tesB deletion
KfadMF		CGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATA$\mathrm{TCCTGGTGTCCCTGTTGATA}$	fadM deletion
KfadmR		TATTCCGGGTGTCGCCGGATGCGGCTTGAGCATCCGGCACCACAAAACGT$\mathrm{ATAGATACATCAGAGCTTTTACGAG}$	fadM deletion
ASFP1		CGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTT$\boxed{\mathrm{CGGTTCTGGCAAATATTCTGAAATG}}$	gene substitution
ASFP2		ATTCCGGGTGTCGCCGGATGCGGCTTGAGCATCCGGCACCACAAAACGTA$\boxed{\mathrm{GCGTTCACCGACAAACAACAGATA}}$	gene substitution
ASFP3		ACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAAT$\mathrm{TCCTGGTGTCCCTGTTGATA}$	gene substitution
ASFP4		ATTCCGGGTGTCGCCGGATGCGGCTTGAGCATCCGGCACCACAAAACGT$\mathrm{ATAGATACATCAGAGCTTTTACGAG}$	gene substitution
Finp1		TTCTAGAGAATAGGAACTTCGGAATAGGAACTAAGGAGGATATTCATATG$\boxed{\mathrm{TCAGGCAGCTTTTTTGCGCTGGCGC}}$	gene insertion
Finp2		AAATATTTTTAGTAGCTTAAATGTGATTCAACATCACTGGAGAAAGTCTT$\boxed{\mathrm{ATGGCAATACAGCAGGTACATCACG}}$	gene insertion
Finp3		AAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATT$\mathrm{TTCCTGGTGTCCCTGTTGATA}$	gene insertion
Finp4		AAATATTTTTAGTAGCTTAAATGTGATTCAACATCACTGGAGAAAGTCTT$\mathrm{ATAGATACATCAGAGCTTTTACGAG}$	gene insertion
TkfadMF		GCACTGCTCATTACCCTGTCCCTG	fadM deletion or substitution
TktesAF		TGCCATGTTCACGGTTGAGGG	tesA deletion test
TktesBF	TTTACCTTCAGTACGCACCGCTTTC		tesB deletion test
TFinF:	GCCGAATATCATGGTGGAAAATGG		gene insertion test
CatR		TGAGCATTCATCAGGCGGGC	fragment integration test
TkfadMR		AGCATCCGGCACCACAAAACG	fadM deletion or substitution
TktesAR		GTCAGGATCTCCGTTGCTTTATGAGTCAT	tesA deletion test
TktesBR		GACGGTTTTCACCTCCGGCTATTT	tesB deletion test
TfinR		CTGGCGATTGCTCCGTCTGC	gene insertion test
KtesAchiF		CCGACGGACTTCTTAAGATGATGAACTTCAACAATGTTTTCCGCTGGCATATGACTCATAAAGCAACGGAGATCCTGACAGGTAAAGTTATGCAAAAATCgctggtgg$\mathrm{TCCTGGTGTCCCTGTTGATA}$	Chi site addtion test
KtesBchiF		ATCACGCATTTCTGCCTGTAATTAGCCCGTAATTCAGACCATTGCACCCAAAAAATAGCCGGAGGTGAAAACCGTCCGGCTGTTTTTTGCAGTGCTTGTTgctggtgg$\mathrm{GTGACGGAAGATCACTTCGCAG}$	Chi site addtion test
KfadMchiF		CGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAgctggtgg$\mathrm{TCCTGGTGTCCCTGTTGATA}$	Chi site addtion test

Note: The bold parts of the primers are homologous 5’ end of the target site; the underlined parts of the primers are homologous 3’ end of the target site; the yellow-highlighted parts of the primers are homologous to cat-sacB cassette; The framed parts of the primers are homologous to the exogenous fragment; the lowercase nucleotides in the KtesAchiF KtesBchiF and KfadMchiF primers are the Chi site.