Table 2. Strain used and constructed in this study.
| Strains | Relevant characteristics | Source |
|---|---|---|
| MG | E. coli K-12 MG1655 | Lab collection |
| MGK1 | MGK ΔfadM:: fadM 3’50-cat-sacB | This study |
| MGK1S | MG ΔfadM | This study |
| MGK2 | MGK1S ΔtesB:: tesB 3’50-cat-sacB | This study |
| MGK2S | MGK1S ΔtesB | This study |
| MGK3 | MGK2S ΔtesA:: tesA 3’50-cat-sacB | This study |
| MGK3S | MGK2S ΔtesA | This study |
| MGKFSF | MG ΔfadM:: FAR- fadM 3’50- cat-sacB | This study |
| MGKFSFS | MG ΔfadM:: FAR | This study |
| MGFARIN | MG ldhA::FAR- ldhA 3’50-cat-sacB | This study |
| MGFARINS | MG ldhA::FAR | This study |
| MGKA | MGK ΔtesA:: tesA 3’50-Chi-cat-sacB | This study |
| MGKAS | MG ΔtesA | This study |
| MGKB | MGK ΔtesB:: tesB 3’50-Chi-cat-sacB | This study |
| MGKBS | MG ΔtesB | This study |
| MGKF | MGK ΔfadM:: fadM 3’50-Chi-cat-sacB | This study |
Note: MG is the original strain used for genome editing.