Table 3. Effects of different procedures on the recombination efficiency (%).

Genome editing	Time point of sucrose addition			Subculture		Inoculation stage
	First subculture	Second subculture	Once	Twice	Thrice	Log phase	Stationary phase	Late stationary phase
ΔfadM	1.9±0.14	5.3±0.47	4.5±0.31	5.4±0.41	6.0±0.27	1.1±0.09	7.3±0.20	8.9±0.41
ΔtesB	2.0±0.09	6.1±0.11	4.2±0.23	5.9±0.13	6.3±0.31	0.9±0.06	8.3±0.32	9.1±0.46
ΔtesA	1.4±0.14	5.5±0.41	4.4±0.29	5.6±0.31	5.9±0.32	0.6±0.08	7.2±0.28	8.3±0.36
ΔfadM:: FAR	2.5±0.17	5.2±0.29	4.1±0.21	5.4±0.19	5.9±0.24	1.1±0.09	7.4±0.27	8.5±0.29
FAR insertion	2.1±0.20	5.0±0.32	4.4±0.31	5.7±0.27	6.2±0.35	1.0±0.05	7.4±0.35	9.1±0.43

Note: Recombination efficiency = number of clonies without cat-sacB/number of total streaked clonies×100%.For the test of time point of sucrose addition, other parameters were kept the same: subculturing twice after sucrose addition and inoculating at the stationary phase. For the test of subculture times, other parameters were kept the same: adding sucrose at the second subculture and inoculating at the stationary phase. For the test of inoculation stage, other parameters were kept the same: adding sucrose at the second subculture and subculturing thrice after sucrose addition, Values are the mean of three biological replicates ± SD