Skip to main content
. 2016 Mar 28;11(3):e0149762. doi: 10.1371/journal.pone.0149762

Table 4. Recombination efficiency with the recommended protocol.

Genome editing ΔfadM ΔtesB ΔtesA ΔfadM:: FAR FAR insertion
Efficiency (%) 5.2± 0.23 5.9±0.35 5.6±0.19 5.6±0.23 6.0±0.30

Note: Parameters for the test: adding sucrose at the second subculture, inoculating at the late stationary phase, subculturing once after sucrose addition. Values are the mean of three biological replicates ± SD