Table 4. Recombination efficiency with the recommended protocol.
Genome editing | ΔfadM | ΔtesB | ΔtesA | ΔfadM:: FAR | FAR insertion |
---|---|---|---|---|---|
Efficiency (%) | 5.2± 0.23 | 5.9±0.35 | 5.6±0.19 | 5.6±0.23 | 6.0±0.30 |
Note: Parameters for the test: adding sucrose at the second subculture, inoculating at the late stationary phase, subculturing once after sucrose addition. Values are the mean of three biological replicates ± SD