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(A) The promoter of PtrWRKY89 was cloned and ligated into vector pCXGUS-P to drive GUS expression and the resulting construct was introduced into A. thaliana. Transgenic seedlings were grown on MS media and then transplanted in soil for GUS staining. GUS expression was observed in various tissues of transgenic plants, including (B) flowers, (C) siliques, (D) 5-week-old seedlings, (E) old leaves, (F) young leaves and (G) roots.
