Table 1.
Day | Isolate | Colony morphologya | Enzyme activityb | Closest phylogenetic memberc | |
---|---|---|---|---|---|
Xylanase | Amylase | ||||
0 | BXP1 | Irregular, white, 2–3 mm | – | + | Bacillus subtilis str. 168 |
0 | BXP7 | Slighly irregular, mucoid, white, 2–3 mm | – | + | Bacillus vallismortis str. DSM 11031 |
1 | BXP11 | Circular, white, 1 mm | + | + | Paenibacillus cookii str. LMG 18419 |
1 | BXP12 | Circular, irregular margin, 1 mm | – | + | Bacillus subtilis str. DSM 10 |
1 | BXP13 | Circular, white, 1–2 mm | – | + | Bacillus subtilis str. 168 |
7 | D7XPN1 | Circular, irregular margin, white, 1 mm | + | + | Bacillus subtilis str. 168 |
16 | D16IS1 | Irregular, filamentous margin, white, 5–6 mm | – | + | Bacillus aerius str. 24 K |
16 | D16IS2 | Circular, mucoid, white, 1.5–2 mm | – | + | Bacillus subtilis str. DSM 10 |
16 | D16IS3 | Rhizoid, white, 4–6 mm | – | + | Bacillus licheniformis str. ATCC 14580 |
16 | D16IS5 | Filamentous, translucent | – | – | Kurthia gibsonii str. NBRC 15534 |
16 | P1XP2 | Circular, mucoid, white, 0.7 mm | + | + | Paenibacillus cookii str. LMG 18419 |
16 | P1XP3 | Irregular, white, 1–3 mm | – | + | Bacillus subtilis str. DSM 10 |
16 | P1XP5 | Circular,mucoid, white, 0.8 mm | + | + | Paenibacillus cookii str. LMG 18419 |
21 | D21IS1 | Circular, mucoid, white, 1–1.2 mm | – | + | Paenibacillus ehimensis str. KCTC 3748 |
21 | D21IS2 | Irregular, slighly opague white, 2 mm | – | + | Bacillus licheniformis str. ATCC 14580 |
21 | D21IS3 | Rhizoid, white, 3 mm | – | + | Bacillus licheniformis str. ATCC 14580 |
21 | D21IS4 | Circular, irregular margin, white 3–4 mm | – | + | Bacillus subtilis str. DSM 10 |
29 | D29IS4 | Circular, mucoid, white, 1 mm | + | + | Paenibacillus cookii str. LMG 18419 |
29 | D29IS5 | Circular, irregular margin, white, 2–3 mm | – | + | Bacillus subtilis str. DSM 10 |
29 | D29IS6 | Irregular, slightly opaque, white, 4–5 mm | + | + | Bacillus licheniformis str. ATCC 14580 |
35 | D35IS1 | Irregular, filamentous margin, white, 6 mm | – | + | Bacillus aerius str. 24 K |
35 | D35IS3 | Irregular, white, 1.2 mm | – | – | Brevibacillus agri str. DSM 6348 |
35 | D35IS5 | Filamentous, translucent, spreading | – | – | Bacillus badius str. 110 |
42 | D42IS3 | Circular, mucoid, white, 1.2 mm | + | + | Paenibacillus cookii str. LMG 18419 |
49 | D49IS3 | Irregular, white, 2–4 mm | – | + | Bacillus subtilis str. DSM 10 |
49 | D49IS4 | Circular, filamentous margin, white, 3–4 mm | – | + | Bacillus licheniformis str. ATCC 14580 |
49 | D49IS5 | Circular, slightly translucent, mucoid, 0.5 mm | – | – | Aneurinibacillus migulanus str. B0270 |
aThe strains were isolated on 0.1 % (dilute) Tryptic Soy Agar (dTSA) medium and colony morphology noted after 48 h incubation at 45 °C
bXylanase and amylase activities were determined as described in “Materials methods” Section
cThe closest phylogenetic match was determined by BLAST analysis of the 16S rRNA sequences of the isolates against the NCBI 16S ribosomal RNA (Bacteria and Archaea) database