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. 2016 Mar 29;10:77. doi: 10.3389/fncel.2016.00077

Figure 2.

Figure 2

Effect of E2 on the spontaneous postsynaptic currents (sPSCs) of GnRH neurons in brain slice of metestrous female mice. (A) E2 in low concentration (10 pM) decreased the frequency of the sPSCs with no change in the average amplitude of them. One-minute-long periods of the recording before and after application of E2 are depicted under the recording. Cumulative probability plot presents reduction in the interevent intervals, but not in amplitude. (B) Pretreatment of the brain slice with the non-selective ER antagonist Faslodex (1 μM, 10 min) inhibited the effect of E2 on the sPSCs. (C) Effect of E2 on the sPSCs was abolished by the pretreatment with cannabinoid receptor type 1 (CB1) inverse agonist AM251 (1 μM, 10 min). (D) Similar inhibition was observed in case of intracellularly applied diacylglycerol (DAG) lipase inhibitor THL (10 μM, 20 min). Individual events of sPSCs show no change in waveform properties in the treated phase (lower insets) as compared to the control phase (upper insets) in all sPSC measurements. Cumulative probability plots in (B–D) graphs present no change in interevent intervals and amplitudes. Arrowhead shows the onset of drug administration.