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. 2016 Mar 29;10:77. doi: 10.3389/fncel.2016.00077

Figure 3.

Figure 3

Effect of E2 on the miniature postsynaptic currents (mPSCs) of GnRH neurons in the presence of non-selective estrogen receptor (ER) antagonist and endocannabinoid receptor/synthesis blockers. (A) E2 decreased the frequency of the mPSCs with no change in the average amplitude of them. One-minute-long periods of the recording before and after application of E2 are illustrated under the recording. Cumulative probability plot presents reduction in the interevent intervals, but not in amplitude in the case of the effect of E2 alone on the mPSCs. (B) Pretreatment of the brain slice with the non-selective ER antagonist Faslodex (1 μM, 10 min) inhibited the action of E2 on the mPSCs. (C) Effect of E2 on the mPSCs was abolished by pretreatment with cannabinoid receptor type 1 (CB1) inverse agonist AM251 (1 μM, 10 min). (D) Similar inhibition was observed in case of intracellularly applied DAG lipase inhibitor THL (10 μM, 20 min). Arrowhead shows the onset of drug administration. Individual events of mPSC in the control phase (upper insets) and the treated phase (lower insets) show no change in waveform properties in any measurements (A—D). Cumulative probability plots of treatments in (B–D) graphs show no change in interevent intervals or amplitudes.