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. 2016 Mar 29;10:63. doi: 10.3389/fncel.2016.00063

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Copyright © 2016 Scholz, Mohrhardt, Jansen, Kalbe, Haering, Klasen, Hatt and Osterloh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental procedure and mixture of agonists. (A) 300-μm thick coronal acute slice through the OE of a transgenic Olfr73-GFP mouse. Scale bar, 100 μm (B) Identification of single GFP-labeled Olfr73 neurons for whole-cell patch clamp measurements. Scale bar, 5 μm (C) Representative patch clamp trace comparing the odor-induced receptor currents elicited by a 1 s application of MIEG (500 μM) or vanillin (100 μM), respectively. (D) Mixtures of strong agonists (vanillin, eugenol) (100/10 μM) with MIEG (500 μM) to evaluate MIEGs' antagonistic potential. Mixtures were applied for 1 s every 30 s and the amplitudes were compared to control measurements (vanillin and eugenol applied separately).