Figure 2.

Inhibition of olfactory cAMP signaling proteins ACIII and CNGC. (Ai) Representative traces showing the odor-induced currents elicited by 1-s application of vanillin (100 μM) or MIEG (500 μm) after 5 min of preincubation with SQ22536 (200 μM) in comparison to the control response prior to SQ22536. (Aii) Quantification of the receptor current amplitudes collected from the AC3 blocking experiments. (Bi) Exemplary traces monitoring the odor-induced receptor currents elicited by vanillin (100 μM)/MIEG (500 μM) using different extracellular conditions (control, 600 nM Ca²⁺, 600 nM Ca²⁺ + NMDG). (Bii) Bar graphs illustrating the mean maximal amplitudes under these different conditions.