TABLE 1.
Result category | Result caused by | Result by functional category of genes (no.) |
Total no. | Total, % | ||||
---|---|---|---|---|---|---|---|---|
Identification | Regulation | Resistance | Virulence | |||||
Concordant (n = 27,119; 96.8 %) | ||||||||
Positive | Microarray and WGS (de novo) | 829 | 990 | 1,060 | 8,495 | 11,374 | 40.6 | |
Negative | Microarray and WGS (de novo) | 0 | 1,159 | 8,100 | 6,486 | 15,745 | 56.2 | |
Discrepant (n = 909; 3.2 %) | ||||||||
False positive | Microarray | Mishybridizations | 0 | 78 | 21 | 103 | 202 | 0.7 |
LFM | Misprediction | 0 | 17 | 2 | 9 | 28 | 0.1 | |
False negative | Microarray | Polymorphisms | 0 | 3 | 14 | 140 | 157 | 0.6 |
LFM | Misprediction | 0 | 0 | 0 | 5 | 5 | <0.1 | |
WGS | Assembly error | 88 | 42 | 16 | 164 | 310 | 1.1 | |
Cropped contig | 1 | 12 | 15 | 28 | 56 | 0.2 | ||
Not sequenced or aberrant allele | 6 | 9 | 8 | 100 | 123 | 0.4 | ||
Unknown | 0 | 0 | 4 | 24 | 28 | 0.1 | ||
Total no. of typing results | 924 | 2,310 | 9,235 | 15,554 | 28,028 | 100 |
Comparison of typing concordance of WGS typing and the traditional DNA microarray. In total, 182 targets in 154 samples were analyzed, resulting in 28,028 individual typing results. The discrepant results are subdivided by false-positive and false-negative typing results and different causes of error. Results that were assumed to be false negatively typed by the two methods are not considered in this table (n = 4).