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. 2016 Mar 25;54(4):1008–1016. doi: 10.1128/JCM.03022-15

TABLE 1.

The percentage of concordantly typed (WGS and microarray identify a gene as present or absent, respectively) and discrepantly typed results (either only WGS or only microarray identifies a gene as present) for each functional target categorya

Result category Result caused by Result by functional category of genes (no.)
Total no. Total, %
Identification Regulation Resistance Virulence
Concordant (n = 27,119; 96.8 %)
    Positive Microarray and WGS (de novo) 829 990 1,060 8,495 11,374 40.6
    Negative Microarray and WGS (de novo) 0 1,159 8,100 6,486 15,745 56.2
Discrepant (n = 909; 3.2 %)
    False positive Microarray Mishybridizations 0 78 21 103 202 0.7
LFM Misprediction 0 17 2 9 28 0.1
    False negative Microarray Polymorphisms 0 3 14 140 157 0.6
LFM Misprediction 0 0 0 5 5 <0.1
WGS Assembly error 88 42 16 164 310 1.1
Cropped contig 1 12 15 28 56 0.2
Not sequenced or aberrant allele 6 9 8 100 123 0.4
Unknown 0 0 4 24 28 0.1
Total no. of typing results 924 2,310 9,235 15,554 28,028 100
a

Comparison of typing concordance of WGS typing and the traditional DNA microarray. In total, 182 targets in 154 samples were analyzed, resulting in 28,028 individual typing results. The discrepant results are subdivided by false-positive and false-negative typing results and different causes of error. Results that were assumed to be false negatively typed by the two methods are not considered in this table (n = 4).