TABLE 1.

Description of primers and probes used in this study

Organism	Target gene	Primer/probe	Sequence (5′ to 3′)	Working concentration (μM)	Reference or source
Human	β-Actin	Forward	CGGCCTTGGAGTGTGTATTAAGTA	0.3	This study
		Reverse	TGCAAAGAACACGGCTAAGTGT	0.3
		Probe	FAM-TCTGAACAGACTCCCCATCCCAAGACC-BHQ	0.2
Streptococcus pneumoniae	lytA	Forward	ACGCAATCTAGCAGATGAAGCA	0.2	17
		Reverse	TCGTGCGTTTTAATTCCAGCT	0.2
		Probe	FAM-TGCCGAAAACGCTTGATACAGGGAG-BHQ1	0.2
Haemophilus influenzae	Hpd	Forward	AGATTGGAAAGAAACACAAGAAAAAGA	0.3	25
		Reverse	CACCATCGGCATATTTAACCACT	0.3
		Probe	FAM-AAACATCCA/ZEN/ATCGTAATTATAGTTTACCCA ATAACCC-3IABkFQ	0.2
Escherichia coli	ompT	Forward	CAAGCCAATGTAGGGCATTTTAA	0.3	This study
		Reverse	TTCAGAGATGATATCGGCTCCTT	0.3
		Probe	FAM-ACGTTGTTTGTAGCCGATTGCTCTTTCTCC-BHQ1	0.2
Neisseria meningitidis	ctrA	Forward	GCTGCGGTAGGTGGTTCAA	0.33	This study
		Reverse	TTGTCGCGGATTTGCAACTA	0.33
		Probe	FAM–TGTGCAGCTGACACGTGGCAATGT–BHQ1	0.2
Streptococcus agalactiae	dltS	Forward	TTTAGGAATACCAGGCGATGAAC	0.3	This study
		Reverse	GCTTTGAATCTTAACCATCTTTTGG	0.3
		Probe	FAM-ATTGCTTTGGTGACTATAG-MGB	0.2
Bordetella pertussis	Porin gene	Forward	TGAACCATGCATACAACCTATTGA	0.33	26
		Reverse	CCTGTCCCCTTAATCCGGAAT	0.33
		Probe	FAM-TCTTCACAGTTAGCCCGCGCGC-BHQ1	0.2
Herpes simplex virus 2	Glycoprotein D gene	Forward	CCACATTCAGCCGAGCCT	0.3	27
		Reverse	CTCGTCCGAAGCCCCG	0.3
		Probe	6FAM-TGTGTACTACGCAGTGCTGGAACGTGC-IABkFQ	0.1
Adenovirus	Hexon gene	Forward	GCCACGGTGGGGTTTCTAAACTT	0.5	28
		Reverse	GCCCCAGTGGTCTTACATGCACATC	0.5
		Probe	FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-3IABkFQ	0.4
Influenza A virus	Segment 7 matrix protein 2 (M2) and matrix protein 1 (M1) genes	Forward	GACCRATCCTGTCACCTCTGAC	0.9	11
		Reverse	AGGGCATTTTGGACAAAKCGTCTA	0.9
		Probe	FAM-TGCAGTCCT/ZEN/CGCTCACTGGGCACG-3IABkFQ	0.25