FIG 2.

(A) Coverage maps of control libraries for LA09-CRF01_AE and LA51-group O. cDNA synthesis (with the corresponding control experiment number in Table 1 given in parentheses) was with oligo(dT)-SMART only (experiment 2), N6 HIV-SMART primers only (experiment 3), N6 HIV-SMART+oligo(dT)-SMART (experiment 4), the same as experiment 4 without the SMART-PCR amplification step (experiment 5), and non-HIV-SMART primers (experiment 6). (B) Neighbor-joining phylogenetic tree of HIV-1 isolate sequences (indicated in red by LA number only); alignment was 8,001 nt in length after gap stripping, and bootstrap values of 100% are shown.