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. 2015 Feb 9;362(3):1–7. doi: 10.1093/femsle/fnu042

Table 2.

Primers.

Primer Sequencea (5 → 3) Use
SpeIACS2Up CGCCACTAGTATGACAATCAAGGAACATAAA ACS2 insertion
ACS2XhoILo GGGGGGCTCGAGTTATTTCTTTTTTTGAGAG ACS2 insertion
ACS2Up TGCTAATCCCGACAAGCCAG ACS2 overexpression verification
AmpUp CCGGCGTCAATACGGGATAA ACS2 overexpression verification
LacLo CCAGCTGGCGTAATAGCGAA ACS2 overexpression verification
Act1-F TGGATTCCGGTGATGGTGTT RT-PCR
Act1-R CGGCCAAATCGATTCTCAA RT-PCR
Acs2-F CTGCTGTTGTCGGTATTCCA RT-PCR
Acs2-R TGTGTTCTGCATCACCTTCA RT-PCR

aThe underlined sequences are added SpeI and XhoI restriction sites.