Table 5.

Genetic modifications reported in non-comprehensive screens of S. cerevisiae mutants that increase acetic acid tolerance.

Target gene	Function	Modification	Strain backgrounda	Reference
ACS2	Acetyl-CoA synthetase	Overexpression	S288c	This study
ATP10	Mitochondrial ATPase	Deletion	W303-1A	Ludovico et al. (2002)
BAP2	Leucine transporter	Complementation of deletion allele	BY4741	Hueso et al. (2012)
CTT1	Cytosolic catalase T	Overexpression	W303-1B	Guaragnella et al. (2008)
CYC3	Cytochrome c	Deletion	W303-1A	Ludovico et al. (2002)
FPS1	Plasma membrane aquaglyceroporin	Deletion	BY4741	Mollapour and Piper (2007)
GCN2	Protein kinase phosphorylates eIF2	Deletion	BY4742	Almeida et al. (2009)
GCN4	Transcriptional activator of amino acid biosynthetic genes	Deletion	BY4742	Almeida et al. (2009)
GLC7′	Type 1 ser/thr protein phosphatase	Overexpression of dominant negative allele	BWG1-7A	Hueso et al. (2012)
HAA1	Transcriptional activator	Overexpression	S288c	Tanaka et al. (2012)
HHF2	Histone 4	Point mutation	H3 WT	Liu, Zhang and Zhang (2014)
HHT2	Histone 3	Point mutation	H3 WT	Liu, Zhang and Zhang (2014)
HIS3	Histidine biosynthesis	Complementation of deletion allele	S288c	Ding et al. (2013)
LEU2	Leucine biosynthesis	Complementation of deletion allele	BWG1-7A	Hueso et al. (2012)
LEU2	Leucine biosynthesis	Complementation of deletion allele	S288c	Ding et al. (2013)
LYS2	Lysine biosynthesis	Complementation of deletion allele	S288c	Ding et al. (2013)
PEP3	CORVET and HOPS complexes	Overexpression	S288c	unpublished data, Ding J, Holzwarth G, Bakalinsky AT (2014)
PPH21	Protein phosphatase 2A subunit	Deletion	BY4742	Almeida et al. (2009)
PPH22	Protein phosphatase 2A subunit	Deletion	BY4742	Almeida et al. (2009)
SIT4	Protein phosphatase 2A-related ser/thr phosphatase	Deletion	BY4742	Almeida et al. (2009)
TOR1	Kinase and rapamycin target	Deletion	BY4742	Almeida et al. (2009)
TRP1	Tryptophan biosynthesis	Complementation of deletion allele	S288c	Ding et al. (2013)
URA3	Uracil biosynthesis	Complementation of deletion allele	S288c	Ding et al. (2013)

^aBY4741: MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0; BY4742: MATa his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0; BWG1-7A: MATa ade1-100 his4-519 ura3-52 leu2-3,112; H3 WT: MATa his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 can1::MFA1pr-HIS3 hht1-hhf1::NatMX4 hht2-hhf2::[HHTS-HHFS]*-URA3 (Baranwal et al., 2014); S288c: MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6; W303-1A: MATa ade2 leu2 his3 trp1 ura3; W303-1B: MATa ade2 leu2 his3 trp1 ura3.