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SEM was used to demonstrate that particles within the expected size range of OMVs (20–500 nm) are present in Synechocystis cultures. (A) SEM of Synechocystis cells. (B) SEM of acellular culture extract showing the presence of particles ∼30 nm in diameter. (C) SEM of buffer confirming that the particles in (B) originate from Synechocystis culture and are not a byproduct of the SEM preparation procedure.
