Table 1.
Transposon mutations from this study influencing erythromycin biosynthesis in S. erythraea.
| Gene | Mutant # | Transposon nucleotide (nt) insertion site | Ery1 | Spo2 | Pig3 | Predicted Function (reference where known) |
|---|---|---|---|---|---|---|
| FL2302 | Control | + | + | Parental strain control | ||
| acrR | S6.18–36 | In SACE_0303; 339,794 | + | + | + | acrR, regulator of multidrug efflux pump (Wu et al. 2014) |
| citA4 | S6.18–32 | In SACE_0632; 696,384 | + | + | + | citA4 citrate synthase (Viollier et al. 2001) |
| hpcH | S7.11–58 | In SACE_0699; 769,528 | + | – | – | hpcH 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase |
| fabG | S6.07–125 | In SACE_0700; 770,333 | + | – | – | fabG, short-chain dehydrogenase/reductase family |
| eryAII | S5.17–06 | In SACE_0723; 802,912 | – | – | – | eryAII erythromycin polyketide synthase |
| cysH | S6.18–04 | In SACE_1474; 1,626,645 | + | + | + | cysH, sulfate adenylyltransferase subunit 2 |
| unk1597 | S6.07–72 | In SACE_1597; 1,752,052 | + | – | +/– | unk1597, hypotheical protein, polar effects expected on SACE_1598 |
| cwh1 | S6.07–03 | In SACE_1598; 1,752,509 | + | – | +/– | cwh1, cell-wall-associated hydrolase, NlpC/P60 superfamily (Anantharaman and Aravind 2003) |
| cccA | S5.17–12 | In SACE_1685; 1,852,856 | – | – | – | cccA - cytochrome c mono-and diheme variants |
| gtf1 | S7.05–53 | Upstream of SACE_2010; 2,197,132 | + | – | – | gtf1, glycosyltransferase- GT1_Gtf_like family (Liang and Qiao 2007) |
| cps2I | S6.15–149 | In SACE_3177; 3,507,747 | + | bld | + | cps2I, nucleotide-sugar-dependent glycosyltransferase, group 1 (Lakkitjaroen et al. 2014) |
| arsC | S6.18–12 | In SACE_5143; 5,750,836 | + | – | – | arsC, arsenical resistance protein, arsenate reductase, arsenic transporter (EC 1.20.4.1) |
| tsp1 | S6.18–17 | In SACE_5967; 6,700,519 | + | + | + | tsp1, secreted trypsin-like serine protease |
| rho1 | S7.12–136 | In SACE_6295; 7,045,657 | + | + | + | rho1, rho1-like transcription terminator (Cardinale et al. 2008) |
| dppII | S6.15–143 | Upstream of SACE_6505; 7,295,927 | + | + | + | dppII, X-Pro dipeptidyl-peptidase (Maes, Scharpé and De Meester 2007) |
1
Erythromycin production phenotype; increased production +, reduced production –, compared to parent strain FL2302.
2
Sporulation phenotype. +, wild type sporulation; –, makes aerial mycelium but no spores; bld, makes no aerial mycelium or spores.
3
Pigmentation. +, normal red pigmentation; –, no red pigmentation; +/–, reduced red pigmentation.