Figure 5.

Inhibition of D. discoideum by M. marinum strains. The virulence of the M. marinum strains was comparatively assessed using a D. discoideum inhibition assay. In this assay, the highest amoeba inoculum inhibited by each strain is considered a measure of its virulence. The fold reduction in virulence of each strain relative to M. marinum WT is shown at the base of each bar for the mutants and above the bar for M. smegmatis (Msm). The results correspond to means and SEM of three experiments. **, Student's t-test P < 0.01 (versus WT).