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. 2016 Mar 29;7:361. doi: 10.3389/fmicb.2016.00361

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Copyright © 2016 Vijayaraghavan, Arun, Vincent, Arasu and Al-Dhabi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Electrophoretic analysis of the crude enzyme from Bacillus sp. IND7 (S- Crude fibrinolytic enzyme; M1-Protein marker [205 kDa – 29 kDa]; M2 – Protein marker [43 kDa-14 kDa]). (B) Zymogram analysis of the crude enzyme on 12% (w/v) sodium dodecyl sulfate – polyacrylamide slab gel (M, Protein marker; S, Crude fibrinolytic enzyme). Two ranges of protein markers (205 kDa – 29 kDa and 43 kDa – 14 kDa) were applied to determine the molecular weight of proteins.