Table 2.
Culture of oral mucosal epithelial cells on temperature-responsive surfaces.
| Author, Year | Type of Study | Cell Suspension/Explant | Substrate | Air-Lifting | Serum | 3T3 | Culture Medium | CultureTime (Days) | Morphology | Phenotype |
|---|---|---|---|---|---|---|---|---|---|---|
| Burillon et al., 2012 [38] | Clinical | Cell suspension | CellSeed a | No | – | Yes | – | – | Similar characteristics to normal corneal epithelium; Basal membrane | Expression of K3/76, p63, laminin 5, and β1-integrin |
| Soma et al., 2014 [69] | Animal | Cell suspension | CellSeed a | – | FBS | Yes | DMEM:F12 (insulin, triiodthyronine, hydrocortisone) | 10–12 | 3–4 cell layers; Stratified epithelium; Cobble stone-like cell morphology | Expression of K14 and p63 |
| Sugiyama et al., 2014 [70] | Animal | Cell suspension | CellSeed a | – | FBS | Yes | DMEM:F12 (penicillin, streptomycin, insulin, cholera toxin, EGF, hydrocortisone, triiodothyronine) | 14 | 3–5 cell layers; Stratified epithelium; Cuboidal cells in the basal layer, squamous epithelium on the apical side | Expression of K4, K13, MUC5 |
| Nishida et al., 2004 [31] | Clinical | Cell suspension | CellSeed a | No | – | Yes | – | 14 | Multilayered cell sheets; Microvilli, desmosomes, basement membrane | Expression of β1-integrin, K3, and p63 |
| Bardag-Gorce et al., 2015 [64] | Animal | Cell suspension | CellSeed a | – | FBS | Yes | – | 14 | Multilayered stratified epithelium | Expression of K4, ΔNp63, TIMP-1, TIMP-3, and connexin 43 |
| Hayashida et al., 2005 [66] | Animal | Cell suspension | CellSeed a | – | FBS | Yes | – | 14 | 3–5 cell layers; Stratified epithelium; | Expression of K3, K4, K13, p63, ΔNp63, and β1-integrin |
| Murakami et al., 2006 [67] | in Vitro | Cell suspension | CellSeed a | – | HAS | No | DMEM/F12 (penicillin, streptomycin, fungizone, transferrin, EGF, cholera toxin, hydrocortisone, triiodothyronine) | 14 | 3–5 cell layers; Cuboidal basal cells, flattened middle cells, and polygonal flattened superficial cells | Expression of p63 and Ki67 |
| Oie et al., 2010 [68] | Clinical | Cell suspension | CellSeed a | – | HAS | Yes | – | 14–17 | 4–5 cell layers; Small basal cells, flattened middle cells, and polygonal flattened superficial cells | Expression of K1, K3/76, K4, K10, K12, K13, K15, ZO-1, and MUC16; Moderate expression of p63 (30.7% ± 7.6% of cells) |