Table 3.
Culture of oral mucosal epithelial cells on other substrates.
| Author, Year | Type of Study | Cell Suspension/Explant | Substrate | Air-Lifting | Serum | 3T3 | Culture Medium | Culture Time (Days) | Morphology | Phenotype |
|---|---|---|---|---|---|---|---|---|---|---|
| Satake et al., 2011 [48] | Clinical | Cell suspension | Fibrin-coated cell culture inserts | Yes | HAS | Yes | DMEM:F12 (penicillin, streptomycin, transferrin, EGF, hydrocortisone, triiodothyronine) | – | 5–6 cell layers; | – |
| Hirayama et al., 2012 [41] | Clinical | Cell suspension | Fibrin-coated cell culture inserts | Yes | HAS | Yes | DMEM:F12 (penicillin, streptomycin, insulin, EGF, hydrocortisone) | – | 5–6 cell layers; | – |
| Sheth et al., 2014 [71] | in Vitro | Explant | Fibrin gel | – | FCS | No | DMEM:F12 (penicillin, cholera toxin, insulin, EGF, hydrocortisone) | - | Multilayered epithelium; Cobblestone morphology | Expression of K3, K13, and K19; High expression of p63 |
| Ilmarinen et al., 2012 [72] | in Vitro | Cell suspension | Collagen IV-coated cell culture inserts | Yes | No | No | Serum-free oral PCT epithelium medium (EGF) | 13–17 | 4–12 cell layers; Stratified epithelium; Cuboidal basal cells and flat intermediate and superficial cells | Expression of K3/12, K4, K13, Ki67, and p63 |
| Kanayama et al., 2007 [34] | in Vitro | Cell suspension | Culture plate | – | FBS | Yes | DMEM (Supplements not reported) | – | Multilayered cells; Normal epithelial morphology | Expression of FGF2, VEGF, Ang1, and TGF-β1 |
| Kanayama et al., 2009 [33] | in Vitro | Cell suspension | Culture plate | – | FBS | Yes | DMEM (Supplements not reported) | 14 | Multilayered cells | Expression of VEGFr-1 |
| Hyun et al., 2014 [74] | Animal | Cell suspension | Culture plate | – | FBS | Yes | DMEM:F12 (penicillin, streptomycin, gentamycin, amphotericin) | 14 | 2–6 cell layers; Stratified epithelium | Expression of K3, K4, and Ki67; High expression of p63 |
| Krishnan et al., 2010 [73] | in Vitro | Explant | Culture plate | – | FBS | – | DMEM:F12 (streptomycin, amphotericin, EGF, insulin, transferrin, selenium, hydrocortisone) | 21 | Multilayered cells; Normal epithelial morphology | Expression of ABCG2, K3, MUC1/4/16, hBD1/2,3; High expression of p63 and ΔNp63 |
ABCG2, ATP binding cassette subfamily G member; Ang1, angiopoietin; DMEM, Dulbecco’s modified eagle medium; EGF, epidermal growth factor; FBS, fetal bovine serum; FCS, fetal calf serum; FGF2, fibroblast growth factor 2; HAS, human autologous serum; hBD, human beta defensing; MUC, mucin; PCT, progenitor cell-targeted; TGF-β1, transforming growth factor beta 1; VEGF, vascular endothelial growth factor; –, indicates not reported.