Table 5.
Overall Effect of Different Culture Methods and Substrates for Cultured Autologous Oral Mucosal Epithelial Cell Sheet.
| Substrate/Method | Air-lifting | Animal-derived Nutrient | Use of 3T3 | Serum-free Medium | Viability | Morphology | Phenotype (Expression of p63) |
|---|---|---|---|---|---|---|---|
| Amniotic membrane | 17/24 | 16/24 | 21/24 | 0/24 | >98% (1) | 4.2 cell layers (15) | ++ |
| Temperature-responsive cell-culture inserts | 0/9 | 5/9 | 8/9 | 0/9 | 86%–93% (2) | 4.3 cell layers (6) | ++ |
| Fibrin-coated culture plate | 2/2 | 0/2 | 2/2 | 0/2 | 5–6 cell layers (2) | – | |
| Fibrin gel | 0/1 | 1/1 | 0/1 | 0/1 | – | – | +++ |
| Collagen IV-coated culture plate | 1/1 | 0/3 | 0/1 | 1/1 | – | 4–12 cell layers (1) | + |
| Culture plate | 0/4 | 4/4 | 3/4 | 0/4 | – | 2–6 cell layers (1) | +++ |
Number of studies using different culture parameters is presented in the Table; –, indicates not reported; +, low expression of p63; ++, moderate expression of p63; +++, high expression of p63.