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. 2016 Apr;147(4):353–368. doi: 10.1085/jgp.201511510

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© 2016 Rodríguez-Banqueri et al.

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Figure 5. — Normalized FSEC profiles of SteT WT, I134V/A377T, and L210Q/M229V solubilized in DDM, DM, Cymal-6, and OG. (A–H) Normalized FSECs of SteT-I134V/A377T (A–D) and SteT-L210Q/M229V (E–H) were overlapped with the corresponding normalized FSEC of SteT WT (dashed lines). SteT variants containing the GFP in the C-terminal end were solubilized from the membrane with DDM (A and E), DM (B and F), Cymal-6 (C and G), or OG (D and H) and injected into the gel filtration column equilibrated with a buffer containing 2× CMC of DDM. RFU, relative fluorescent units.