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. 2016 Apr;147(4):353–368. doi: 10.1085/jgp.201511510

Figure 8.

Figure 8.

SEC profiles of DDM-purified SteT WT, I134V/A377T, and L210Q/M229V. Purified proteins from His-tag affinity chromatography were concentrated up to 2 mg/ml and injected in a Superdex 200 50/1 50G column. (A and B) Monodispersity in these experimental conditions was analyzed by overlapping the normalized chromatograms of SteT WT with I134V/A377T (A) and L210Q/M229V (B).