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. 2016 Apr;147(4):337–351. doi: 10.1085/jgp.201511551

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© 2016 Li et al.

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Figure 5. — Mutational effects of the BKγ1 subunit on its association with the BKα subunit in the isolated channel complex. Immunoprecipitation and immunoblot analyses of the BK channel complexes in the presence of the γ1 wild type or different mutants (indicated on top). BKα and γ1 were both tagged with V5 at their C termini, cotranslationally expressed in HEK-293 cells, immunoprecipitated with anti-BKα antibody, and then immunoblotted with anti-V5 antibody.