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. 2016 Mar 2;5(1):9. doi: 10.3390/cells5010009

Figure 3.

Figure 3

Cellular localization of TbBILBO1 in procyclic cells: (A–D) Micrographs of a PF cytoskeleton labeled for immunofluorescence. The cell has duplicated its FPC and the daughter is located in the posterior rounded end of the cytoskeleton. The cytoskeleton was probed with monoclonal anti-TbBILBO1 and DAPI. (A) DAPI; (B) monoclonal anti-TbBILBO1 (white arrowheads); (C) merged DAPI, anti-TbBILBO1; and (D) merged DAPI, anti-TbBILBO1 and Phase contrast. Scale bar: 5 μm. (E–G) Electron micrographs of immunogold-labeled thin sections through the T. brucei FPC (black arrowheads). The FPC was labeled after detergent extraction using a monoclonal anti-TbBILBO1 antibody followed by 10 nm gold-conjugated anti-mouse antibodies. Micrographs (E,F) are longitudinal sections of the FPC and (G) is a longitudinal section of the axoneme (BB, basal body). Asterisks indicate the axoneme traversing the FPC. Scale bar: 250 nm.