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. 2016 Mar 2;5(1):9. doi: 10.3390/cells5010009

Figure 4.

Figure 4

The effects of mutated TbBILBO1 expression in a mammalian system and in T. brucei. (A) Immunofluorescence labeling of TbBILBO1 carrying a mutation in EF hand 2 (mEFH2:myc) expressed for six hours in U-2 OS cells and probed using anti-NTD antibody. This mutated protein produces helical and ring shaped polymers. The inset shows a higher magnification of the boxed region. Scale bar: 10 μm; scale bar in magnified inset: 1 μm; (B) Immunofluorescence labeling of a T. brucei procyclic cytoskeleton expressing TbBILBO1 carrying a mutation in EF hand 1 (mEFH1:myc). This mutation induces helical polymers. Cells were probed with anti-NTD antibody (green) and anti-myc (red) after 24 h of induction, and the images were merged. The inset shows the anti-NTD label alone. Scale bar: 5 μm; scale bar in magnified inset: 1 μm. Reproduced from [44].