Figure 1.

RASSF10 promoter structure and normal expression levels (a) The RASSF10 promoter with its CpG island structure. Black vertical lines represent single CpGs and restriction enzyme TaqI recognition sites are marked. Bent arrow indicates transcriptional start site (TSS). Horizontal arrows mark PCR products. PCR product size was 241 bp for COBRA methylation analysis. Restriction site positions for COBRA are shown. Pyrosequencing covers seven CpGs (asterisks) within the COBRA analysed region. 100 bp standard is shown; (b) RASSF10 expression is shown relative to b-Actin expression in human breast, kidney, liver, lung and heart RNA samples of healthy donors after reverse transcriptase PCR and quantitative PCR.