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. 2016 Feb 25;8(3):26. doi: 10.3390/cancers8030026

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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RASSF10 inhibits growth of breast cancer cell lines. (a) Colony formation analyses were performed in breast cancer cell lines MCF7, T47D and ZR75-1 after empty vector and RASSF10 overexpression. Cells were selected for three weeks using G418 and were Giemsa stained for visualization; (b) Quantification of colony formation in breast cancer cell lines MCF7 (p < 0.005) and T47D (p < 0.05) shows significant inhibition of colony growth under RASSF10. Statistically significant (asterisks) by paired T-Test, two tailed; (c) Overexpression verification of RASSF10 in MCF7 and T47D cell lines by RT-PCR.