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. 2016 Feb 25;8(3):26. doi: 10.3390/cancers8030026

Figure 5.

Figure 5

RASSF10 induces apoptosis in breast cancer cell lines. Overexpression of EYFP or RASSF10-EYFP in breast cancer cell lines induces morphological changes and DNA fragmentation associated with apoptosis induction. (a) Breast cancer cell lines MCF7, T47D and ZR75-1 exhibit alteration of nuclei shape after RASSF10 overexpression. Cells were analysed 72 h post transfection according to nuclei shape as normal, condensed, fragmented or missing (n = 100); (b) MCF7 cells show reduced nuclei size after RASSF10-EYFP transfections (5 μm standard) and (c) according nuclei measurements are shown (n = 17); (d and e) RASSF10 overexpression for 48 h or 72 h increases sub G1 fraction of transfected cells in flow cytometry analysis (n > 8000) using propidium iodide staining of DNA.