. 2016 Mar 3;8(3):31. doi: 10.3390/cancers8030031

Table 2.

Differentially expressed miRNAs based on SOLiD sequencing data. The selection of putative mRNA targets is based on publications in the field of cancer and cell proliferation, not on target prediction tools.

miRNA	Change Related to	* Fold Change	Selected 10 Putative Target mRNA for Analysis by RT-qPCR
let-miR-7g	II/N	+10.48	Cyclin D, E2F, p53 [24]
miR-122¹	II/N	−81.56	Cyclin G1 [25]
miR-17	II/N	−10.34	Cyclin D1, E2F1, PTEN [26]
miR-130a	II/N	+6.32
miR-143	IA/N	−5.17
miR-148b	II/N	−6.02
miR-152	II/N	+6.31	E2F3, RICTOR [27]
miR-193b	IA/N	−5.76	RUNX1T1 (Cyclin D-related) [28], (Cyclin D1) [29]
miR-199a-5p	IA/N	−6.20	E2F3 [30]
miR-21	IA/II:	−5.45	PTEN [31]
miR-218	II/N	+8.55	RICTOR [32,33], mTOR [32], regulating p53 [34]
miR-218	IA/N	+7.59	RICTOR [32,33], mTOR [32], regulating p53 [34]
miR-26b	IA/II	+8.86	Activation of PTEN–Akt pathway [35]
miR-34a	IA/II	−8.51	Sirt1 [36]
miR-342-3p	IA/N	+7.52
miR-376c	II/N	+8.14	ALK7 [37]
miR-376c	IA/II	−7.92	ALK7 [37]
miR-424	II/N	+6.33
miR-451	IA/N	−10.07	PI3K/Akt/mTOR signaling pathway [38]
miR-451	IA/II	−6.90	PI3K/Akt/mTOR signaling pathway [38]
miR-574-3p	IA/II	+13.12	P63 [39]
miR-99a	IA/II	−6.09	mTOR [40,41]
miR-99a	II/N	+5.42	mTOR [40,41]

¹: This large drop of expression was not found in any other tumor sample in a preliminary RT-qPCR and was not included in further validation experiments. *: Fold change ±5.17 folds, selected for validation by RT-qPCR.