Figure 4.

HDAC6 deletion results in increased levels of acetylated tubulin within the superficial umbrella cell and smooth muscle layers of the bladder. (A) Western blots showing levels of α-tubulin and acetylated α-tubulin in the mucosal layers of WT and HDAC6^−/− bladders; (B) Fluorescent micrographs of bladder sections from WT and HDAC6^−/− mice stained as indicated to visualize acetylated α-tubulin (green), uroplakin III (red), and nuclei (blue). Arrowheads highlight examples of acetylated α-tubulin within the smooth muscle layers. L, lumen. Scale bars = 100 µm.