FIGURE 5.

Involvement of α7nAChR in the effect of Ani on extracellular K⁺ in C2C12 cells. C2C12 myotubes were pretreated for 1 h with mecamylamine (Mec, 10 μmol/L), MLA (10 μmol/L), or hexamethonium (Hex, 10 μmol/L), and then for 30 min with Ani (500 μg/ml), ACh (100 μmol/L), Ani (500 μg/ml) + ACh (100 μmol/L), nicotine (0.01–10 μmol/L), or PNU282987 (0.01–10 μmol/L) prior to exposure to KCl (5 mmol/L), and extracellular K⁺ and glucose levels were measured 6 h later. Extracellular K⁺ and glucose in cultured myotubes were significantly decreased by Ani + ACh (A,B), nicotine (C,D), and PNU (I,J). Mec and MLA, but not Hex significantly counteracted the effects of Ani + ACh (E,F) or nicotine (G,H). N = 8 per group. ^∗P < 0.05, ^∗∗P < 0.01. Power = 1.00 for (A–J).