FIGURE 7.

Influences of insulin signaling blocking on the effect of Ani on extracellular K⁺ in C2C12 cells. C2C12 myotubes were pretreated for 1 h with HNMPA-(AM)3 (HNMPA, 200 μmol/L), LY 294002 (10 μmol/L), rapamycin (200 nmol/L), stattic (10 μmol/L), or ouabain (2 mmol/L), and then for 30 min with Ani (500 μg/ml) + ACh (100 μmol/L) prior to exposure to KCl (5 mmol/L), and extracellular K⁺ and glucose levels were measured 6 h later. Extracellular K⁺ and glucose in cultured myotubes were significantly decreased by Ani + ACh. HNMPA (A,B), LY 294002 (C,D), and rapamycin (E,F) attenuated such effects. Stattic (G,H) and ouabain (I,J) attenuated the effect of Ani + ACh on extracellular K⁺ but not on glucose. N = 8 per group. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. ACh + Ani. Power = 1.00 for (A–J).