Table 1.
Protein basic local alignment search tool (BLASTp) searches for vertebrate immunoregulatory receptor-like sequences and intracellular signaling proteins in the Channel catfish (Ictalurus punctatus).
| Human FcRLs | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
|---|---|---|---|---|---|---|
| FcRL6 (XP_005245185) | FcRI (NP_001187150) | 4e-09 | 55.8 | 26% | 37% | 89% |
| FcRL5 (AAK31327) | LITR TS32.15 L2.1a (ABI23564) | 1e-19 | 165 | 26% | 40% | 90% |
| FcRL4 (NP_112572) | LITR TS32.15 L2.2c (ABI23567) | 3e-11 | 63.2 | 25% | 38% | 89% |
| FcRL3 (NP_443171) | LITR TS32.15 L1.1a (ABI16036) | 1e-18 | 88.2 | 25% | 39% | 90% |
| FcRL2 (NP_110391) | LITR TS32.17 L2.1a (ABI23578) | 6e-06 | 46.2 | 24% | 39% | 79% |
| FcRL1 (NP_443170) | Hepacam 2 (NP_00118775) | 2e-04 | 40.8 | 23% | 46% | 38% |
| Human FcRs | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| FcyRI (NP_000557) | FcRI (NP_001187150) | 2e-17 | 79.0 | 26% | 40% | 96% |
| LITR TS32.15 L1.1a (ABI16036) | 2e-11 | 62.4 | 26% | 40% | 50% | |
| FcεRIα (XP_003821022) | LITR TS32.17 L1.1a (ABI16049) | 2e-07 | 48.5 | 29% | 43% | 96% |
| FcγRIIA (NP_001129691) | −no matches− | − | − | − | − | − |
| FcγRIIIA (NP_001121068) | LITR TS32.15 L2.1a (ABI23564) | 1e-07 | 49.7 | 25% | 44% | 77% |
| FcγRIIC (NP_963857) | −no matches− | − | − | − | − | − |
| FcγRIIIB (NP_001231682) | LITR TS32.15 L2.1a (ABI23564) | 1e-05 | 44.3 | 25% | 44% | 66% |
| FcγRIIB (NP_001002274) | −no matches− | − | − | − | − | − |
| FcµR (AAP36498) | −no matches− | − | − | − | − | − |
| PIGR (NP_002635) | −no matches− | − | − | − | − | − |
| FcαµR (NP_114418) | −no matches− | − | − | − | − | − |
| Human LRC Proteins | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| FcαR (NP_001991) | −no matches− | − | − | − | − | − |
| NKp46 (O76036) | −no matches− | − | − | − | − | − |
| LAIR1 (NP_001275954) | −no matches− | − | − | − | − | − |
| LILRB1 (AAC51179) | LITR1 (AAW82352) | 6e-10 | 98.2 | 25% | 39% | 66% |
| LILRB2 (XP_011546934) | LITR TS32.15 L2.2c (ABI23567) | 9e-13 | 68.6 | 23% | 38% | 85% |
| LILRB3 (O75022) | LITR1 (AAW82352) | 4e-07 | 50.4 | 23% | 39% | 73% |
| LILRB4 (NP_001265355) | −no matches− | − | − | − | − | − |
| LILRB5 (NP_006831) | LITR1 (AAW82352) | 2e-10 | 60.8 | 24% | 38% | 66% |
| LILRA1 (NP_006854) | LITR TS32.15 L2.2c (ABI23567) | 2e-10 | 61.2 | 24% | 38% | 89% |
| LILRA2 (XP_011545392) | LITR1 (AAW82352) | 2e-13 | 70.1 | 24% | 38% | 66% |
| LILRA3 (Q8N6C8) | LITR TS32.17 L1.2b (ABII6052) | 4e-09 | 55.5 | 24% | 40% | 53% |
| LILRA4 (P59901) | LITR1 (AAW82352) | 2e-08 | 54.7 | 26% | 40% | 65% |
| LILRA5 (A6NI73) | −no matches− | − | − | − | − | − |
| LILRA6 (AGZ61988) | LITR1 (AAW82352) | 1e-09 | 51.2 | 23% | 38% | 68% |
| KIR3DL1 (ADM64608) | LITR3 (NP_001187136) | 7e-08 | 52.4 | 27% | 41% | 65% |
| KIR3DS1 (ABX88987) | LITR3 (NP_001187136) | 8e-07 | 48.9 | 27% | 41% | 65% |
| KIR2DL1 (AAC50335) | −no matches− | − | − | − | − | − |
| KIR2DS1 (XP_011546300) | −no matches− | − | − | − | − | − |
| KIR2DL4 (ABW73959) | −no matches− | − | − | − | − | − |
| Human Siglecs | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| Siglec-1 (Q9BZZ2) | LITR3 (NP_001187136) | 2e-16 | 137 | 25% | 39% | 51% |
| Siglec-2 (NP_001762) | LITR TS32.15 L1.1a (ABI16036) | 7e-18 | 170 | 26% | 40% | 74% |
| Siglec-3 (P20138) | −no matches− | − | − | − | − | − |
| Siglec-4 (NP_002352) | −no matches− | − | − | − | − | − |
| Siglec-15 (Q6ZMC9) | −no matches− | − | − | − | − | − |
| Human CEACAMs | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| Ceacam-1 (NP_001703) | Hepacam 2 (NP_00118775) | 4e-14 | 144 | 31% | 44% | 64% |
| LITR3 (NP_001187136) | 1e-09 | 58.5 | 24% | 43% | 65% | |
| Ceacam-3 (NP_291021) | Hepacam 2 (NP_00118775) | 9e-11 | 59.3 | 31% | 50% | 56% |
| Ceacam-4 (NP_001808) | −no matches− | − | − | − | − | − |
| Ceacam-5 (NP_001295327) | LITR3 (NP_001187136) | 1e-22 | 166 | 25% | 41% | 75% |
| Ceacam-6 (P40199) | Hepacam 2 (NP_00118775) | 4e-14 | 71.2 | 31% | 47% | 48% |
| LITR TS32.17 L2.2b (ABI23581) | 3e-09 | 56.2 | 27% | 41% | 56% | |
| Ceacam-7 (Q14002) | Hepacam 2 (NP_00118775) | 2e-07 | 49.7 | 36% | 52% | 32% |
| Ceacam-8 (NP_001807) | Hepacam 2 (NP_00118775) | 6e-10 | 58.5 | 27% | 42% | 51% |
| LITR TS32.15 L2.3b (ABI23569) | 2e-08 | 53.1 | 28% | 42% | 56% | |
| Mouse PIRs | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| PIRA (NP_035217) | LITR TS32.15 L1.1a (ABI16036) | 1e-12 | 61.6 | 24% | 39% | 88% |
| PIRB (AAC53219) | LITR TS32.15 L1.1a (ABI16036) | 1e-11 | 105 | 24% | 39% | 92% |
| Chicken Receptors | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| FcRL1-like (XP_015135539) | LITR TS32.17 L2.1a (ABI23578) | 2e-06 | 46.6 | 26% | 43% | 73% |
| FcRL2-like (NP_001090998) | IpFcRI (NP_001187150) | 4e-21 | 91.3 | 24% | 43% | 65% |
| LITR3 (NP_001187136) | 3e-11 | 63.2 | 25% | 41% | 69% | |
| CHIR-A (AAG37067) | LITR TS32.17 L2.1a (ABI23578) | 5e-09 | 53.9 | 28% | 45% | 75% |
| CHIR-B (AAG37068) | LITR1 (AAW82352) | 6e-10 | 101 | 27% | 42% | 83% |
| CHIR-AB1 (NP_001139613) | LITR TS32.15 L2.2c (ABI23567) | 3e-07 | 79.7 | 30% | 46% | 78% |
| Xenopus Receptors | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| FcγRI-L (XP_012809801) | FcRI (NP_001187150) | 1e-18 | 84.0 | 34% | 45% | 63% |
| LITR TS32.15 L2.2c (ABI23567) | 1e-12 | 67.4 | 28% | 45% | 62% | |
| FcR3-L (XP_012824905) | FcRI (NP_001187150) | 6e-17 | 80.1 | 22% | 46% | 43% |
| LITR TS32.15 L2.3c (ABI23570) | 2e-15 | 76.6 | 25% | 43% | 61% | |
| FcR4-L (XP_012809008) | FcRI (NP_001187150) | 4e-18 | 83.2 | 21% | 44% | 58% |
| LITR TS32.15 L2.2c (ABI23567) | 6e-15 | 75.5 | 23% | 40% | 92% | |
| FcR5-L (XP_012825305) | LITR TS32.17 L2.1a (ABI23578) | 8e-19 | 89.7 | 25% | 44% | 41% |
| ILR-1 (XP_002938564) | LITR TS32.17 L2.2b (ABI23581) | 2e-12 | 102 | 28% | 44% | 67% |
| ILR-2 (NP_001121201) | LITR TS32.17 L2.1a (ABI23578) | 3e-12 | 65.1 | 20% | 45% | 69% |
| Human Signaling Proteins | Top Matching Catfish Sequences | E value | Score | ID | Pos. | Coverage |
| c-Src (P12931) | Lymphocyte PTP (AJW77401) | 0.0 | 560 | 59% | 77% | 82% |
| SYK (P43405) | SYK (NP_998008) * | 0.0 | 863 | 67% | 77% | 98% |
| PI3K p85α (NP_852664) | PI3K p85α (AHH41763) | 0.0 | 864 | 62% | 76% | 99% |
| Csk (NP_004374) | Csk-like (AJW77401) | 3e-105 | 318 | 40% | 58% | 95% |
| GRB2 (CAG467401) | GRB2 (NP_001187313) | 3e-151 | 421 | 91% | 95% | 100% |
| Gab2 (BAA76737) | Gab2 (XP_692935) | 0.0 | 793 | 63% | 75% | 99% |
| Nck1 (NP_001278928) | Nck1 (NP_001278928) * | 0.0 | 615 | 77% | 87% | 100% |
| SHP-1 (AAA36610) | SHP-1-like (XP_009290704) * | 0.0 | 783 | 66% | 78% | 95% |
| SHP-2 (BAA02740) | SHP-2-like (CBX19678) * | 0.0 | 1076 | 91% | 94% | 100% |
| SHIP-1 (AAB49680) | SHIP-1 (AJK26904) | 0.0 | 1164 | 56% | 67% | 99% |
| SH2D1A (NP_001108409) | SH2D1A (NP_001187495) | 2e-49 | 155 | 64% | 76% | 89% |
| Vav3 (EAW51251) | Vav3 (XP_009296581) * | 0.0 | 1173 | 68% | 80% | 100% |
| Rac1 (NP_008839) | Rac1 (AD027935) | 4e-136 | 381 | 94% | 98% | 100% |
| Cdc42 (AAM21109) | Cdc42 (NP_001188177) | 2e-134 | 377 | 96% | 97% | 100% |
| RhoA (P61586) | RhoA-like (NP_001187623) | 2e-138 | 387 | 95% | 98% | 100% |
| Wave2 (P61586) | Wave3 (NP_001074059) * | 1e-104 | 322 | 44% | 53% | 100% |
| Wave2 (NP_957375) * | 2e-104 | 313 | 76% | 85% | 38% | |
| WASp (NP_000368) | WASp (NP_956232) * | 1e-106 | 327 | 54% | 69% | 59% |
| N-WASp (BAA20128) | N-WASp (NP_001076475) * | 3e-145 | 428 | 75% | 85% | 53% |
(A) The amino acid sequences listed in the left column were used as queries to search the non-redundant protein sequence database for catfishes (taxid:7995) by blastp at http://blast.ncbi.nlm.nih.gov/Blast; (B) Other than the human signaling protein queries, all searches we performed using the predicted extracellular regions of the receptor sequences (i.e., predicted TM segments and CYT regions were excluded from the searches); (C) For each search result the Expect (E) value reported provides the overall significance of the match with lower values closer to zero being considered more significant. The score indicates quality of the alignment with a higher score associated with a better alignment. This value is calculated using a formula that considers alignment of similar or identical residues, as wells as gaps in the alignment; (D) The top matching Channel catfish sequences are listed in the second column from the left and those marked with an * represent queries that did not retrieve a match using catfishes (taxid:7995) but did retrieve a match using Danio (taxid:7954); (E) Only sequences with scores >40.0 are reported.