Table 2.
Protein BLAST searches for human immunoregulatory receptor-like sequences in teleost fishes.
| Human Receptors | Teleost Matches | E value | Score | ID | Pos. | Coverage |
|---|---|---|---|---|---|---|
| FcRL5 (AAK31327) | Zebrafish CD22 (XP_009293714) | 3e-46 | 184 | 27% | 43% | 94% |
| Salmon Sialoadhesin-like (XP_009293614) | 3e-46 | 184 | 26% | 45% | 97% | |
| Herring Sialoadhesin-like (XP_012675080) | 8e-33 | 316 | 30% | 45% | 96% | |
| Cichlid CD22-like (XP_014269289) | 1e-32 | 276 | 28% | 47% | 76% | |
| Herring FcRL5 (XP_012678524) | 4e-31 | 261 | 25% | 40% | 94% | |
| FcγRI (NP_000557) | Asian arowana FcRL5 (KPP56756) | 6e-21 | 98.2 | 29% | 44% | 91% |
| Trout unamed protien (CDQ78931) | 2e-18 | 88.6 | 27% | 44% | 81% | |
| Salmon FcγRI-like (ACN10126) | 4e-18 | 88.2 | 28% | 42% | 81% | |
| Catfish FcRI (NP_001187150) | 3e-15 | 79.0 | 26% | 40% | 96% | |
| Catfish TS32.15 L1.1a (ABI16036) | 4e-09 | 62.4 | 29% | 44% | 50% | |
| FcγRIIA (NP_001129691) * | Herring FcRL5 (XP_012678524) | 7e-10 | 208 | 31% | 41% | 79% |
| Yellow Croaker FcγRII-like (XP_010752023) | 9e-10 | 60.5 | 25% | 45% | 78% | |
| Tilapia FcγRIB-like (XP_013119926) | 2e-09 | 59.7 | 37% | 54% | 41% | |
| Mexican tetra CD22 (XP_015459483) | 3e-09 | 60.8 | 31% | 47% | 70% | |
| Herring FcRL3 (XP_012675232) | 2e-08 | 58.9 | 27% | 50% | 70% | |
| FcγRIIB (NP_001002274) * | Yellow Croaker FcγRII-like (XP_010752023) | 1e-11 | 65.9 | 28% | 48% | 67% |
| Killifish unknown protein (XP_013889184) | 2e-10 | 65.1 | 27% | 43% | 82% | |
| Mexican tetra CD22 (XP_015459483) | 4e-10 | 63.9 | 31% | 46% | 61% | |
| Herring FcRL3 (XP_012675232) | 2e-09 | 62.0 | 28% | 51% | 70% | |
| Cichlid FcγRII-like (XP_006808904) | 3e-08 | 58.2 | 28% | 42% | 76% | |
| FcµR (AAP36498) * | Damselfish unknown protein (XP_008286766) | 4e-06 | 52.8 | 34% | 47% | 42% |
| Salmon CMRF35-like (XP_014058627) | 8e-06 | 51.2 | 34% | 47% | 42% | |
| Killifish PIGR-like (012722396) | 2e-05 | 50.1 | 29% | 41% | 40% | |
| PIGR (NP_002635) * | Tilapia PIGR-like (XP_013123119) | 4e-25 | 115 | 27% | 44% | 67% |
| Yellow Croaker PIGR (KKF27361) | 1e-23 | 111 | 25% | 39% | 81% | |
| Medaka PIGR (XP_011484914) | 3e-23 | 262 | 28% | 44% | 72% | |
| Carp PIGR (ADB97624) | 1e-18 | 92.8 | 31% | 47% | 73% | |
| Zebrafish PIGR (NP_001289179) | 7e-18 | 90.5 | 30% | 47% | 68% | |
| FcαR (NP_001991) * | Cichlid unknown protein (XP_014265321) | 7e-05 | 48.5 | 29% | 46% | 51% |
| NKp46 (O76036) * | Atlantic molly CD276-like (XP_014823336) | 4e-05 | 49.7 | 24% | 37% | 65% |
| Sailfin molly IgSF 1-like Rc (XP_014882122) | 4e-04 | 46.6 | 24% | 38% | 69% | |
| LAIR1 (NP_001275954) * | −no matches− | − | − | − | − | − |
| LILRB1 (AAC51179) | Pike DSCAM-like (XP_12987993) | 1e-15 | 84.3 | 28% | 41% | 85% |
| Pike FcRL5 (XP_010867787) | 6e-14 | 79.7 | 26% | 43% | 82% | |
| Salmon IgSF 1-like Rc (XP_01401166) | 2e-11 | 71.6 | 27% | 41% | 83% | |
| Pike LILRA4-like (XP_012988338) | 4e-10 | 64.3 | 28% | 44% | 42% | |
| Cafish LITR1 (AAW82352) | 2e-09 | 65.1 | 26% | 40% | 86% | |
| LILRB4 (NP_001265355) * | Cichlid IgSF 1-like Rc (XP_014267965) | 2e-11 | 68.9 | 27% | 47% | 78% |
| Amazon molly PEACAM-like (XP_007571377) | 4e-07 | 56.2 | 28% | 42% | 77% | |
| Atlantic molly IgSF 1-like Rc (XP_014827576) | 1e-05 | 51.6 | 29% | 45% | 78% | |
| Yellow Croaker LILRA2-like (KKF09113) | 2e-05 | 51.2 | 31% | 45% | 64% | |
| LILRA2 (XP_011545392) | Trout unamed protien (CDQ78931) | 1e-12 | 75.9 | 27% | 42% | 90% |
| Pike DSCAM-like (XP_12987993) | 5e-12 | 73.6 | 27% | 42% | 82% | |
| Catfish LITR1 (AAW82352) | 4e-11 | 70.1 | 24% | 38% | 66% | |
| Mexican tetra FcRL5 (XP_015464057) | 8e-11 | 69.7 | 28% | 41% | 72% | |
| Pike FcRL5 (XP_010867787) | 7e-10 | 66.7 | 27% | 42% | 77% | |
| LILRA5 (A6NI73) * | Pllatyfish IgSF 1-like Rc (XP_005816340) | 4e-06 | 53.1 | 28% | 40% | 72% |
| Cichlid KIR2DL4-like (XP_005951820) | 4e-05 | 48.9 | 35% | 48% | 40% | |
| Cichlid FcγRI-like (XP_014267764) | 6e-05 | 48.5 | 34% | 49% | 40% | |
| Cichlid IgSF 1-like Rc (XP_014267965) | 3e-04 | 47.0 | 32% | 45% | 44% | |
| KIR3DL1 (ADM64608) | Asian arowana FcRL5 (KPP56756) | 1e-11 | 70.5 | 25% | 42% | 63% |
| Salmon KIR3DL1-like (XP_014042396) | 2e-11 | 69.7 | 30% | 43% | 85% | |
| Cichlid IgSF 1-like Rc (XP_014267965) | 9e-08 | 70.5 | 25% | 43% | 85% | |
| Salmon KIR3DS1-like (XP_014043811) | 2e-07 | 55.5 | 31% | 44% | 56% | |
| Catfish LITR3 (NP_001187136) | 1e-05 | 52.0 | 27% | 41% | 65% | |
| KIR2DL1 (AAC50335) * | Salmon KIR3DS1-like (XP_014043811) | 1e-05 | 49.7 | 28% | 40% | 78% |
| Salmon KIR3DL1-like (XP_014042396) | 4e-05 | 49.3 | 28% | 40% | 78% | |
| Salmon LILRA4-like (XP_014037968) | 8e-05 | 48.5 | 29% | 41% | 75% | |
| Pike OSCAR-like (XP_012988534) | 1e-04 | 47.8 | 29% | 44% | 81% | |
| Pike DSCAM-like (XP_12987993) | 1e-04 | 48.1 | 26% | 43% | 81% | |
| Siglec-2 (NP_001762) | Zebrafish CD22 (XP_009293714) | 8e-71 | 738 | 30% | 45% | 99% |
| Cichlid CD22-like (XP_005755784) | 4e-64 | 221 | 33% | 52% | 67% | |
| Tilapia CD22-like (XP_013132285) | 6e-61 | 218 | 30% | 46% | 85% | |
| Trout unamed protien (CDQ84455) | 2e-58 | 212 | 31% | 47% | 86% | |
| Siglec-4 (NP_002352) * | Salmon Siglec-4 (MAG) (XP_014021122) | 4e-154 | 459 | 44% | 64% | 96% |
| Pike Siglec-4 (MAG) (XP_014021122) | 7e-152 | 454 | 43% | 63% | 96% | |
| Black cod Siglec-4 (MAG) (XP_010788688) | 4e-151 | 452 | 44% | 63% | 96% | |
| Tiger puffer Siglec-4 (MAG) (NP_001027876) | 2e-149 | 449 | 43% | 63% | 96% | |
| Ceacam-3 (NP_291021) | Herring Hemicentin-1-like (XP_012676907) | 1e-18 | 90.5 | 34% | 52% | 65% |
| Cichlid CEACAM 5-like (XP_014266060) | 4e-16 | 82.8 | 33% | 53% | 82% | |
| Salmon CEACAM 1-like (XP_014038441) | 1e-15 | 79.3 | 33% | 52% | 55% | |
| Medaka CEACAM 1-like (XP_011483887) | 5e-15 | 77.8 | 28% | 42% | 94% | |
| Ceacam-4 (NP_001808) * | −no matches− | − | − | − | − | − |
| Ceacam-5 (NP_001295327) | Tongue sole CEACAM 5-like (XP_008322222) | 4e-80 | 277 | 30% | 50% | 85% |
| Yellow Croaker CEACAM 5 (KKF27703) | 4e-75 | 918 | 32% | 53% | 98% | |
| Pike CEACAM 5 (XP_012994902) | 4e-69 | 570 | 32% | 48% | 86% | |
| Cichlid Hemicentin-1-like (XP_004550953) | 1e-65 | 892 | 30% | 47% | 83% | |
| Salmon Hemicentin-1-like (XP_014056825) | 3e-65 | 1041 | 30% | 46% | 86% |
(A) The human receptor amino acid sequences listed in the left column were used as queries to search the non-redundant protein sequence database for teleost fishes (taxid:32443) by blastp at http://blast.ncbi.nlm.nih.gov/Blast; (B) Human receptor sequences marked with an * indicate those that did not retrieve matches by blastp searches using catfishes (taxid:7995); see Table 1; (C) All searches we performed using the predicted extracellular regions of the receptor sequences (i.e., predicted TM segments and CYT regions were excluded from the searches); (D) For each search result the Expect (E) value reported provides the overall significance of the match with lower values closer to zero being considered more significant. The score indicates quality of the alignment with a higher score associated with a better alignment. This value is calculated using a formula that considers alignment of similar or identical residues, as wells as gaps in the alignment; (E) Representative top-matching teleost protein sequences are listed in the second column from the left; (F) Only sequences with scores >40.0 are reported.