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. 2016 Mar 3;8(3):63. doi: 10.3390/toxins8030063

Figure 2.

Figure 2

Recombinant expression strategy of Tgu6.1. (A) Full precursor structure of Tgu6.1. The signal sequence is shaded in gray; the pro-region is underlined; and the mature peptide is shaded in blue. (B) Schematic representation of Tgu6.1 fusion protein. The fusion protein was expressed under the control of a pET-32a T7 promoter and contains thioredoxin as the fusion partner, His6-tag for purification and the enterokinase (EK) site for the cleavage of Tgu6.1 from TRX by enterokinase. (C) Plasmid map of the expression vector. The Tgu6.1 gene was cloned into pET-32a XA/LIC plasmid by ligation independent cloning.