Table 1.
(a) Number of genes and codon usage for alanine codons in human cells. (b) Wobble pair rules reviewed in [30]. (c) Analysis of ERWT and ERAla87 codon-anticodon recognition by alanine tRNAs restricted to tRNA Ala sequences described in the literature according to Modomics [31,32,33]. * indicates that recognition is not frequent in cell context. ↓ indicates low affinity recognition.
| a. Alanine tRNA Genes in Human Genome According to the Genomic tRNA Database [29] | ||||
| Anticodon (5′ → 3′) | Corresponding Codon (5’ → 3’) | nº of Genes | Genome Codon Usage | |
| AGC | GCT | 30 | 1.84 | |
| GGC | GCC | 1 | 2.77 | |
| CGC | GCG | 5 | 0.74 | |
| UGC | GCA | 10 | 1.58 | |
| b. Wobble Pair Rules Reviewed in [30] | ||||
| tRNA 5’ Anticodon Base | mRNA 3’ Codon Base | ER Ala 87 Polymorphism Recognized by tRNA | ||
| G | U,C | Ala87 | ||
| C | G | wt | ||
| k2C | A | --- | ||
| A | U,C,G>A | wt, Ala87 | ||
| U | U,A,G>C | wt, Ala87 ↓ | ||
| xm5s2U,xm5Um,Um,xm5U | A>G | wt ↓ | ||
| xo5U | U,A,G | wt | ||
| I | A,C,U | Ala87 | ||
| c. Analysis of ERWT and ERAla87 Codon-Anticodon Recognition by Alanine tRNAs Restricted to tRNA Ala Sequences Described in the Literature According to Modomics [31,32,33]. | ||||
| tRNA-AGC: | -without modifications recognizes: wt *, Ala87 * | |||
| -modified A → I recognizes: Ala87 | ||||
| tRNA-CGC: | -without modifications recognizes: wt * | |||
| tRNA-UGC: | -without modifications recognizes: wt, Ala87 ↓ | |||
| -with modifications recognizes: wt | ||||
| tRNA-UGC: | -without modifications recognizes: Ala87 | |||