Table 4.
Synthetic life systems.
| Type * | Insertion | Altered Site | System | Ref. |
|---|---|---|---|---|
| o-Synthetic | NCAA | Proteome-wide |
B. subtilis LC33, LC88, E.coli B7-3: 4FTrp, 5FTrp, 6FTrp; E. coli MT16-20: [3,2]Tpa |
[178,180,186,187,188,189] |
| m-Synthetic | NCAA | Proteome-wide | B. subtilis HR15, HR23: 4FTrp | [178,180] |
| o-Synthetic | NCAA | Specific sites | E. coli, C. elegans etc.: p-aminoPhe, p-azidoPhe etc. | [191,192,193,194,195,196,197,198,199,200,201,202,203,204,205,206,207,208] |
| m-Synthetic | NCAA | Specific sites |
E. coli C321ΔA: biphenylPhe etc.; E. coli thyA R126L: azaLeu |
[209,210,211] |
| o-Synthetic | NCDN | Genome-wide | E.coli CLU5: 5-chloroU | [212,214] |
| m-Synthetic | NCDN | Genome-wide | E. coli CLU5 variant: 5-chloroU | [213] |
* o-synthetic = optional synthetic; m-synthetic = mandatory synthetic.