Figure 4.

A non-covalent interaction of HSV-1 gN with gM is sufficient for its transport to the TGN. The subcellular localizations of gN–EYFP, gN–C46A–EYFP, HA–gM or HA–gM–C59A were analyzed alone, alternatively, combinations of either wild type with the corresponding mutant or of both mutants were analyzed by transient expression in HeLa cells for 20 h. Anti-HA antibodies were used to detect the localization of HA–gM and HA–gM–C59A followed by secondary reagents. gN–EYFP and gN–C46A–EYFP were visualized directly. Nuclei were visualized by DAPI staining. The scale bar corresponds to 10 µm.