Table 1.

Primer sequences used for plasmid cloning and BAC mutagenesis.

Primer Name	Primer Sequence
attB_5′	ggggacaagtttgtacaaaaaagcaggct
attB_3′	ggggaccactttgtacaagaaagctgggt
UL49.5–FL_5′	aaaaagcaggctccgccatgggcccccccagaag
UL49.5–FL_3′	agaaagctgggtctaggcgtgcccggcagc
UL49.5–Y2H_3′
UL49.5–Y2H_5′	aaaaagcaggctccgccatgccgcgcggggagccg
UL49.5–IF_3′	agaaagctgggtggcgtgcccggcagc
UL10–FL_5′	aaaaagcaggctccgccatgggacgcccggcccccagag
UL10–Y2H_5′
UL10–Y2H_5′–N
UL10–FL_3′	agaaagctgggtctaccaacggcggacggtgc
UL10–Y2H_3′
UL10–Y2H_3′–C
UL10–∆C_3′	agaaagctgggtctaggtgcagcggagcacggccatgc
UL10–Y2H_3′–N
UL10–C_5′	aaaaagcaggctccgccatgcgcgcctatctgtatcac
UL10–Y2H_5′–C
UL10–M19I_5′	cagaggatctcccgactccgcgccccccacgaaaggcataaccggggcgcggac
UL10stop362aa_5′	gcgcatgcgcgactagcgacaccgcgcac
UL10stop362aa_3′	gtgcgcggtgtcgctagtcgcgcatgcgc
UL10stop423aa_5′	ggggagccgatttaggacgaggtggcg
UL10stop423aa_3′	cgccacctcgtcctaaatcggctcccc
UL10stop434aa_5′	cgaccaaaccgacgtatagtacgccaagatacaacac
UL10stop434aa_3′	gtgttgtatcttggcgtactatacgtcggtttggtcg
gM–C59A_5′	cacggtttcccgccttttacgccacggcg
gM–C59A_3′	gtggcgtaaaaggcgggaaaccgtgcccg
gN–C46A_5′	gggcgcgcgggccgagacccaaaacactg
gN–C46A_3′	gttttgggtctcggcccgcgcgcccccgatc
H5–gM/gk	tccgcgctagcgatacgctcgacgtgtactgttcgcactcgtcgtccccacctgttgacaattaatcatcggca
H3–gM/gk	caccacggtcgggttaaacacaaacggtttattaaaacggaaccaaacaggccagtgttacaaccaattaacc
UL10–mt1_5′	gcgctagcgatacgctcgacgtgtactgttcgcactcgtcgtccccaatgggatgaccggcccccagaggatctcccg
UL10–mt1_3′	caccacggtcgggttaaacacaaacggtttattaaaacggaaccaaacagctaccaacggcggacggtgc
H5–gM–mt3/gK	gccatgccgcacgccacgctgatcgccggaaacgtctgctcttggttgctcctgttgacaattaatcatcggca
H5–UL49.5/gK	cccaacacatagcaggccgcgggcccggcgtccgcgtggagcatgcggagggcctgttgacaattaatcatcggca
H3–UL49.5/gK	aagtcctgggacaccctccacccccacccctcaccccacacagggcgggtgccagtgttacaaccaattaacc
ΔUL49.5_5′	cccaacacatagcaggccgcgggcccggcgtccgcgtggagcatgcggagggatgggccccccctagtaggtct
ΔUL49.5_3′	aagtcctgggacaccctccacccccacccctcaccccacacagggcgggttcaggcgtgcccggcagccagt